FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007925960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449663
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66101.4699897478778552No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG39740.8837729588603022No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38870.8644251361575224No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG31700.7049723904346143No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG30820.6854021789651361No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG27200.6048974454202369No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC23890.5312867636429949No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG21700.4825836237359978No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC19040.4234282117941659No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17680.39318333952315404No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG17590.39118184062286643No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17400.38695645405559276No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG17130.38095195735473014No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG16590.3689429639530048No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16360.36382802231893663No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15480.3442578108494584No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG13070.2906621180750918No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG12620.28065462357365406No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12610.28043223480695545No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12600.28020984604025684No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG11710.26041724580407993No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11550.2568590255369021No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10830.24084703433460167No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG10700.23795598036751966No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG10510.23373059380024597No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10250.2279484858660819No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9960.22149921163182204No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9940.22105443409842482No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG9670.2150499373975622No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC9570.21282604973057603No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8880.19748122482837147No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8780.1952573371613853No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8230.1830259549929614No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7940.17657668075870153No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7940.17657668075870153No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7340.16323335475678452No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7310.1625661884566887No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6720.1494452512214703No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG6380.14188403315371734No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6160.13699148028634778No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5780.12854070715180035No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5650.12564965318471835No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5440.12097948908404738No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5430.12075710031734876No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5260.11697649128347229No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5120.11386304854969166No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG5040.11208393841610272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)