FastQCFastQC Report
Wed 31 May 2023
EGAF00007925976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468516
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62631.3367739842395991No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA20070.42837384422303615No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG19900.42474536622015047No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16030.3421441316838699No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14910.3182388648413288No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14870.31738510531123804No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13600.2902782402308566No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13130.28024656575229023No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12070.2576219382048852No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12000.2561278590272264No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9530.20340820804412232No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9470.20212756874898619No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG8970.19145557462285173No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8920.1903883752102383No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC8170.17438038402103662No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8010.17096534590067364No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7900.16861750719292404No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7870.16797718754535598No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7660.16349495001237951No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7600.16221431071724338No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7020.14983479753092743No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6840.14599287964551905No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC6660.14215096176011063No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6510.1389493635222703No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6080.1297714485737947No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6000.1280639295136132No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5780.12336825209811404No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5780.12336825209811404No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5710.12187417292045521No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5390.11504409667972919No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5340.11397689726711574No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5300.113123137737025No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC5240.11184249844188887No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4910.10479898231864013No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4840.10330490314098131No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG4790.10223770372836787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)