FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491423
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20910.4254990100178461No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18770.3819520046884252No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18040.36709718511343586No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14830.30177667711930456No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14330.29160214316383243No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11220.22831654196079548No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10290.20939190880361727No Hit
AACAGTCTGAATTGCCGCATCCACAGTGGAAACCGGCGGTGGGG10040.20430464182588115No Hit
GACGGTCTGGATAGCCGCGTCTACGGTGGACACCGGCGGCGGGG9560.1945370892286279No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8640.1758159467505591No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT8440.17174613316837023No Hit
ACCGGTCAGCGGGTAGCGGAATTTTGCATCAACTACTTCCGCGT8320.1693042450190569No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8100.16482745007864913No Hit
CACAGTCTGAATTGCTGCATCAACAGTGGATACCGGCGGCGGCG7970.16218207125022638No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7940.16157159921289804No Hit
CTGCGGTGGGAAACCCGGACGCGGTGCCATACCCGGGTGCGGCG7690.15648433223516195No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7570.15404244408584866No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7050.14346092877215758No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7030.1430539474139387No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6320.12860610919716822No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6120.12453629561497935No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6060.12331535154032269No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6000.12209440746566602No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5430.11049543875642776No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5110.10398373702492558No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5100.10378024634581613No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5070.1031697743084878No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4930.10032090480095558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)