FastQCFastQC Report
Wed 31 May 2023
EGAF00007925995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494069
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21140.4278754586909925No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18370.37181041514444335No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18100.3663455914052491No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14980.30319651708567025No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14560.2946956801580346No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10980.22223616539390248No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10460.2117113196739727No Hit
AACAGTCTGAATTGCCGCATCCACAGTGGAAACCGGCGGTGGGG10340.20928250912321963No Hit
GACGGTCTGGATAGCCGCGTCTACGGTGGACACCGGCGGCGGGG10060.2036152845047959No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9150.1851968044949187No Hit
ACCGGTCAGCGGGTAGCGGAATTTTGCATCAACTACTTCCGCGT8880.1797319807557244No Hit
CACAGTCTGAATTGCTGCATCAACAGTGGATACCGGCGGCGGCG8630.17467195877498892No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8470.1714335447073182No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8290.16779032888118867No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8230.16657592360581214No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7890.15969429371201188No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7670.15524147436896465No Hit
CTGCGGTGGGAAACCCGGACGCGGTGCCATACCCGGGTGCGGCG7260.14694303832055847No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7030.1422878180982818No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6680.13520378732525215No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6270.12690535127684593No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5900.11941651874535743No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5830.1179997125907515No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5550.11233248797232775No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5470.11071328093849239No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5240.10605806071621576No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5130.10383165104469214No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5000.10120043961470969No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT4990.10099803873548027No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4950.10018843521856259No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG4950.10018843521856259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)