FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442468
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA17980.4063570698897999No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA15590.35234186427041053No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15050.3401375918710506No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14600.3299673648715839No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14510.3279333194716906No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12720.2874784165182567No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11960.2703020331413797No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG9830.2221629586772377No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9610.21719084769972066No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG9330.21086270645560812No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG8960.20250051981160222No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8750.19775441387851778No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8380.18939222723451188No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8300.18758418687905115No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8260.18668016670132076No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG8140.18396810616812967No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8040.18170805572380377No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8030.18148205067937115No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7380.1667917227912527No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6190.13989712250377428No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5760.13017890559317286No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5470.12362475930462768No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5280.11933066346040844No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5270.11910465841597584No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5240.11842664328267807No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5230.11820063823824546No Hit
TGGACGACCGCCGCCGCGACCACGACCGCGGCCACGACCGCGAC5030.11368053734959364No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5030.11368053734959364No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5020.11345453230516105No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4950.1118724969941329No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4800.10848242132764405No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4710.10644837592775072No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4570.10328430530569441No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4490.1014762649502337No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4470.1010242548613685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)