FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465727
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCTCGCCACGAGAACACCTCTCAGGTTC46591.0003714622514905No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT33700.7235998771812672No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23640.5075935043491144No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG19280.4139764282508852No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19010.4081790405108572No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG18400.3950812385796829No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC16620.35686142310838753No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16580.3560025508506056No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15490.33259828182604834No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA15420.33109525537492995No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15000.32207709666821976No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA14550.3124147837681732No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC14040.3014641624814537No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13990.3003905721592263No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT12320.26453265539683124No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC11960.2568028050767939No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11240.24134310443671936No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT11190.240269514114492No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10690.22953361089221797No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA10240.2198712979921714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9670.20763236831877904No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9580.2056999057387697No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9340.2005466721920782No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG9220.19797005541873244No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA9040.1941051302587138No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8780.18852246058313132No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8670.18616056187423102No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8650.18573112574534006No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8640.1855164076808946No Hit
CTGCCGAAACTGGCAGCAAACCGTGAGAAAC8320.17864542961863925No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8250.1771424031675209No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG8000.17177445155638388No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA7870.16898311671859265No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG7830.16812424446081073No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT7260.15588531478741838No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7220.15502644252963646No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7150.1535234160785181No Hit
AAATACAACCTGCAGGTCCGCGGCACCCGTG7060.1515909534985088No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT7030.15094679930517232No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6970.14965849091849948No Hit
ACCAAAGCCGCTATCAGCCAGCTGGAAAAAC6800.1460082838229263No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6800.1460082838229263No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6780.14557884769403534No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6620.14214335866290767No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6390.13720484318066165No Hit
TACAACCTGCAGGTGCGCGGCACTGCCGGTG6220.13355463608508847No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6140.13183689156952463No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG6060.1301191470539608No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5990.12861612060284244No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5950.1277572483450605No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5950.1277572483450605No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5920.1271130941517241No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5790.12432175931393284No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5720.12281873286281449No Hit
AAATACCACCTGCAGGTTCGTGGCACCCGTG5720.12281873286281449No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5650.12131570641169612No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC5540.11895380770279584No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5530.11873908963835036No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5510.1183096535094594No Hit
AAACTGGAGGAAGTTGCCGGTAAATACTCCC5440.11680662705834105No Hit
AAGTTCAACCTGCAGGTTCGTGGTACCCGCG5440.11680662705834105No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5420.11637719092945009No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5410.11616247286500461No Hit
AACCAGAAAGTCGACGTTCTGACCAAAGCAG5350.11487416447833172No Hit
AACGACGGTCAGGAAAGCGAGCAGAACACCT5290.11358585609165886No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5270.11315641996276787No Hit
CGTTACAACCTGCAGGTCCGTGGCACTGCCG5250.11272698383387693No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5170.11100923931831308No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA5150.11057980318942212No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5100.10950621286719474No Hit
AAAGCGGCTCTGCTGGCCCAGGAAGGCGCAA5020.10778846835163089No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4900.10521185157828514No Hit
AAAAAGGTTAAAGCTGACCCGGAAGCAGTTA4860.1043529793205032No Hit
CTGCAGGTCCGCGGCACCCGTGGTGAACACA4810.10327938899827582No Hit
CACCTGCAGGTGCGTGGTACTCGTGGTGAAC4790.10284995286938485No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4730.10156164448271197No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4720.10134692641826651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)