FastQCFastQC Report
Wed 31 May 2023
EGAF00007926254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417830
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCTCGCCACGAGAACACCTCTCAGGTTC41050.9824569801115287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40440.967857741186607No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31630.7570064380250341No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20680.49493813273340836No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17800.4260105784649259No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17300.4140439891822033No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15920.3810162027618888No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG15030.3597156738386425No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT14650.3506210659837733No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC14450.3458344302706843No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14230.3405691309862863No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13330.31902927027738553No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13240.31687528420649547No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA12400.2967714142115214No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT11910.2850441567144532No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11910.2850441567144532No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC11110.26589761386209704No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT11090.2654189502907881No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10210.24435775315319627No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9500.22736519637173014No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9020.21587727066031637No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8680.20773998994806497No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA8470.2027140224493215No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8090.1936194145944523No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8030.1921834238805256No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG8000.19146542852356221No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7810.1869181245961276No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7430.1778235167412584No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7000.16753224995811694No Hit
CTGCCGAAACTGGCAGCAAACCGTGAGAAAC6870.16442093674460906No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG6860.1641816049589546No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6850.16394227317330015No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA6770.16202761888806452No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6480.15508699710408538No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6450.15436900174712204No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6400.15317234281884978No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6330.1514970203192686No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6200.1483857071057607No Hit
ACCAAAGCCGCTATCAGCCAGCTGGAAAAAC6150.14718904817748846No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6050.14479573032094392No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6030.14431706674963501No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5910.1414450853217816No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5890.1409664217504727No Hit
AAATACAACCTGCAGGTCCGCGGCACCCGTG5830.13953043103654597No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG5770.13809444032261925No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5610.13426513175214802No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5570.1333078046095302No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5490.13139315032429458No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5420.12971782782471342No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5410.12947849603905895No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5390.12899983246775004No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5350.12804250532513223No Hit
AACGACGGTCAGGAAAGCGAGCAGAACACCT5310.1270851781825144No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5310.1270851781825144No Hit
AAATACCACCTGCAGGTTCGTGGCACCCGTG5270.12612785103989663No Hit
TACAACCTGCAGGTGCGCGGCACTGCCGGTG5190.124213196754661No Hit
AAGTTCAACCTGCAGGTTCGTGGTACCCGCG5130.12277720604073428No Hit
AACCAGAAAGTCGACGTTCTGACCAAAGCAG5030.12038388818418976No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC4990.11942656104157194No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4930.11799057032764522No Hit
AAACTGGAGGAAGTTGCCGGTAAATACTCCC4810.11511858889979179No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4810.11511858889979179No Hit
AAAGCGGCTCTGCTGGCCCAGGAAGGCGCAA4700.11248593925759282No Hit
CGTTACAACCTGCAGGTCCGTGGCACTGCCG4650.11128928032932053No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA4640.11104994854366608No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4620.11057128497235717No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG4490.10745997175884928No Hit
CTGCAGGTCCGCGGCACCCGTGGTGAACACA4430.10602398104492257No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4350.10410932675968694No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4240.10147667711748797No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4200.10051934997487015No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4180.10004068640356124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)