FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007926256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502392
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA80351.5993487157438813No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT42980.8555072533002118No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA41090.8178872275036226No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35970.7159747766684182No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32630.6494928263188904No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22750.452833643847832No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22330.44447363811525664No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20480.4076498033408175No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16840.3351964203251644No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15110.30076115861717545No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13510.26891351773117406No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13310.26493256262042386No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12870.2561744613767735No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11740.23368206500103506No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11640.23169158744565996No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10600.2109906208697591No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10520.20939823882545902No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10220.20342680615933373No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9940.1978534690042835No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9820.1954648959378334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9130.18173060080574532No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8560.17038487874010733No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8520.1695886877179573No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8480.16879249669580726No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG8450.16819535342919473No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8050.1602334432076944No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7820.1556553448303317No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7310.14550390929791876No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7130.1419210496982436No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6860.13654676029873086No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6620.13176961416583066No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6250.12440484721094286No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6180.1230115129221803No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6130.12201627414449275No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5930.11803531903374258No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5760.11465150718960493No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5580.11106864758992976No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5530.11007340881224223No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5350.10649054921256708No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5140.1023105463462794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)