FastQCFastQC Report
Sun 21 May 2023
EGAF00007926258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478745
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA75901.5853951477300023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44990.9397487180022767No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT41460.8660142664675349No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37490.7830891184242134No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA33970.7095635463555755No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30140.6295627108377111No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20950.4376024814880573No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20470.42757626711506125No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19320.40355512851309155No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16200.3383847350886171No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14120.2949378061389675No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12920.26987227020647736No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12640.264023645155563No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12200.25483294864698325No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10860.2268431001890359No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10240.21389257329058267No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10130.21159489916343774No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9820.2051196357142111No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9770.2040752383836907No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9480.19801773386667224No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8960.18715600162925983No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8020.16752133181547588No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7820.1633437424933942No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7630.1593750326374166No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7560.15791287637468798No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7520.15707735851027163No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7080.14788666200169193No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7010.14642450573896332No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6920.14454459054402657No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6470.13514501456934275No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6350.13263846097609375No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5810.12135896980647318No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5720.1194790546115364No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5720.1194790546115364No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG5620.11739025995049558No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5370.11216827329789344No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5360.11195939383178938No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5260.10987059917074851No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5230.10924396077243628No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5120.10694628664529134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)