FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007926270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479610
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA55691.1611517691457642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45270.9438919121786451No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30720.6405204228435604No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26050.5431496424177977No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25950.5410646149996873No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23550.4910239569650341No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14780.308167052396739No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG14700.3064990304622506No Hit
ATGGCTCAGCGTAACGGTATGTCCCCGCGTC11480.23936114759909094No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11340.23644210921373618No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA10740.22393194470507288No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC10310.21496632680719752No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC10240.21350680761452012No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG9880.20600070890932215No Hit
TCCGTGAAAAGCGGCGAACACGAGCTGATCG9540.1989116156877463No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA8800.18348241279372823No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG8640.18014636892475136No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA8050.16784470715789912No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7940.16555117699797753No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7860.16388315506348908No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7840.163466149579867No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7440.1551260399074248No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG7410.15450053168199163No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7060.14720293571860468No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6900.14386689184962784No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA6810.14199036717332833No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6710.13990533975521777No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC6680.13927983152978463No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6630.13823731782072934No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC6450.13448426846813036No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC5940.12385062863576655No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5790.12072308750860075No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5780.12051458476678967No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG5620.11717854089781281No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5550.11571902170513543No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5470.11405099977064698No Hit
GAAGCGGAAGCGTGGGAACGTCGTGACGGCG5420.1130084860615917No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5180.10800442025812639No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4830.10070682429473947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)