FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007926272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511913
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA58641.1455071467221971No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31110.6077204524987645No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29030.5670885482494096No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28940.5653304370078509No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25130.49090372778186914No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG16350.31939020888315006No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15300.2988789110649661No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12240.2391031288519729No Hit
ATGGCTCAGCGTAACGGTATGTCCCCGCGTC12220.23871243746495988No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA11320.22113132504937363No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC11060.21605233701820428No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG10870.2123407688415805No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC10820.21136404037404793No Hit
TCCGTGAAAAGCGGCGAACACGAGCTGATCG10360.2023781384727483No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA9720.1898760140883314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9620.18792255715326628No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9310.18186684065456435No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8630.1685833334961214No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG8510.16623918517404324No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8430.16467641962599114No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA8200.1601834686753413No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG8190.1599881229818348No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA8150.15920674020780876No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7730.15100222108053515No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC7680.1500254926130026No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7380.1441651218078072No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA7280.14221166487274206No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7150.13967217085715736No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7140.13947682516365084No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC6930.13537456560001407No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC6650.12990488618183166No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6460.12619331800520792No Hit
GAAGCGGAAGCGTGGGAACGTCGTGACGGCG6400.12502124384416882No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6320.12345847829611672No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6240.12189571274806461No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6070.11857483595845388No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5740.11212842807273891No Hit
GTGACCGACCTGATGGCACTGGAAGACTCTG5660.11056566252468682No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5530.10802616850910214No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC5370.1049006374129979No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5340.10431460033247839No Hit
CCAGTTACGCTGAACAGCGCCGCACCGGCTT5340.10431460033247839No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5170.10099372354286763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)