FastQCFastQC Report
Sun 21 May 2023
EGAF00007926279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527904
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT55071.0431820937140086No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC47940.9081196581196582No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA36840.6978541553009638No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA30610.5798402739892102No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT26910.5097517730496455No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT24910.4718660968660969No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA24010.45481754258350005No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT23380.44288355458568224No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA22210.4207204340183064No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT20440.3871916105958659No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT16100.30497969327756563No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA15300.28982542280414625No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG14830.2809222889010123No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14690.2782702915681639No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG13280.25156088985876224No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA12530.2373537612899315No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10540.19965751348730074No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10180.19283809177426198No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9100.17237982663514578No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8990.17029611444505063No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8700.16480269139843606No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC8630.16347669273201187No Hit
GTGCAGGGACAGATCCAGCGGTGCATCTGGGTCCTCGTAACGTG8410.15930926835182155No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8280.1568466993998909No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC7870.14908013578226345No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT7850.14870127902042796No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7730.14642813844941505No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7550.14301842759289568No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7270.1377144329271989No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7250.1373355761653634No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7150.13544129235618596No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6950.13165272473783113No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6790.12862187064314723No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA6270.11877159483542463No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6270.11877159483542463No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6010.11384645693156331No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5990.11346760016972782No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5930.11233102988422138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)