FastQCFastQC Report
Sun 21 May 2023
EGAF00007926292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428461
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA81281.8970221327028598No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA40840.9531789357724507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39350.9184033085858457No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35600.8308807569417053No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26610.6210600264668197No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18050.4212752152471287No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15680.36596096260803196No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15040.3510237804607654No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14970.3493900261634081No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13990.3265174660004061No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13890.3241835312898957No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12040.2810057391454531No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11330.26443480270082925No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10400.24272920989308247No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10260.23946170129836788No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9760.22779202774581586No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC9510.2219571909695398No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9180.21425520642485546No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG9120.2128548455985492No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8840.20631982840912005No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8660.20211874593020132No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8550.1995514177486399No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7760.18111333353560768No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7600.177379037998791No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7420.17317795551987228No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7420.17317795551987228No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA7240.16897687304095355No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6170.14400377163849218No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5930.1384023283332672No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5730.1337344589122464No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5550.12953337643332763No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG5160.12043103106233706No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4990.11646334205446937No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4980.11622994858341833No Hit
ACCTACACCTACGACGGTAACGGTAACGTGA4960.11576316164131624No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4870.11366262040185687No Hit
CTGTCCGGTCAGCAGTTCCTGAAATGTCCAC4800.1120288661044996No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4700.1096949313939892No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4610.10759439015452983No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4550.10619402932822357No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA4470.10432688155981523No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4420.10315991420456003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)