FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432246
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA82051.8982246220901986No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA40780.9434442423990043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37110.858538887577907No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA36340.8407249575473227No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27280.6311220925121342No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18220.42151922747694603No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16160.3738611809016162No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15100.3493381083919805No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14990.3467932612447541No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14160.32759123277022806No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13950.3227328882164323No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11060.25587281316657645No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10960.253559315760007No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10510.2431485774304447No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9940.22996164221299906No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG9770.2260286966218311No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9740.22533464739986028No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9710.22464059817788942No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8880.20543856970336336No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8730.20196832359350927No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC8700.20127427437153844No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8400.1943337821518302No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7890.18253494537832624No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7750.17929604900912907No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA7320.1693480101608806No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7260.16795991171693897No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7030.16263886768182934No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6640.15361622779620865No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6220.14389953868861713No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5960.13788444543153666No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5170.11960781591963834No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5140.11891376669766754No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4950.11451812162518565No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4890.11313002318124402No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4790.1108165257746746No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG4690.1085030283681052No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4680.10827167862744827No Hit
CTGTCCGGTCAGCAGTTCCTGAAATGTCCAC4490.10387603355496638No Hit
ACCTACACCTACGACGGTAACGGTAACGTGA4330.10017443770445533No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA4330.10017443770445533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)