FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446071
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT74821.6773114593865102No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15410.34546070020243413No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG14030.3145239210798281No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC13620.3053325591665901No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11920.2672220341604812No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT11050.2477184125397078No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10840.24301064180365906No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10670.23919958930304816No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10560.23673361415559407No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC10460.2344918185669994No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG8960.20086488473807979No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8250.18494813605905788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8160.1829305200293227No Hit
ATGGCCCGTCACCACAACATTAACGGTAACA7430.1665654122325818No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7310.16387525752626825No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7110.15939166634907895No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7010.1571498707604843No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6850.1535629978187329No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6670.14952776575926255No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6110.13697371046313256No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5900.1322659397270838No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5810.13024832369734862No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5760.1291274259030513No Hit
AGCGATGACAAACTGGAACGTAACTGCTACT5610.12576473252015935No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5450.12217785957840792No Hit
CTGGGCTGGAAAGCTGGTATCCTGACCGGTT5310.11903934575437543No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5000.11208977942973204No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4920.11029634295885633No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4470.10020826281018043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)