FastQCFastQC Report
Sun 21 May 2023
EGAF00007926317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438003
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG61851.4120907847663142No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG47041.073965246813378No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG39610.9043317054906016No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG39050.8915464049332996No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27290.623055093229955No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23090.5271653390501891No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19440.4438325764892021No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG19430.4436042675506789No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG17630.4025086586164935No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG16770.3828740899034938No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14050.3207740586251692No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12290.28059168544507684No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG11780.268947929580391No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT9960.22739570276915913No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT8530.19474752456033406No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8340.19040965472839227No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7640.17442802903176463No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA6750.15410853350319517No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6670.15228206199500918No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6660.1520537530564859No Hit
ATCGCCGGTCGGGGTGCCGGTGGTCGGCTTCACCTGGACCAGCA6480.1479441921630674No Hit
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA6440.14703095640897437No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6390.1458894117163581No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6290.14360632233112558No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5560.12693976981892818No Hit
ACCGACATTTAAATCCGCGGCTCACATAGTGTCGCCCGGTACTA5480.12511329831074217No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG5260.12009050166323061No Hit
TTCTTCGTCAGAGTCCTTCAGGTGACGGTAACCGTTTTTGCGGT5210.11894895697061436No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5120.11689417652390509No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG5010.1143827782001493No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT4710.10753351004445175No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4710.10753351004445175No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA4670.10662027429035874No Hit
AGTGATAACGTAACGGGTTTCGATCATGTCTTCCGGCTGCACGG4660.10639196535183548No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4620.10547872959774249No Hit
TTTGGCAACAACCTGTACTGGCGGCACCGGCGGCGGGCTCAGGT4560.10410887596660297No Hit
GACTAAATTTAAATCCCTGTTTCACATGGTATCGCCCGGAACTA4540.10365225808955647No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG4510.1029673312739867No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG4480.10228240445841695No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG4460.10182578658137044No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4450.10159747764284718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)