FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007926318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438003
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACAGAAACAGCTGCTGCAGGGTGACG59941.3684837775083731No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT48431.10570018926811No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG46171.0541023691618552No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG42570.9719111512934843No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG36560.8346974792410098No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27570.6294477435086061No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG23520.536982623406689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20960.4785355351447364No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG19710.44999691782933No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18640.42556786140734193No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14760.33698399326032014No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13420.30639059549820435No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG12060.2753405798590421No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC10170.2321901904781474No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA9920.2264824670150661No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9700.22145967036755454No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8610.19657399606852008No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8380.19132289048248527No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7450.17009015919982284No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7420.1694052323842531No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6930.15821809439661372No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6750.15410853350319517No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6640.1515971351794394No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6620.15114051730239292No Hit
ACGGCAGCGACTTCCAAAGCCGTTGCTGCCA6620.15114051730239292No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG6250.14269308657703259No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5740.1310493307123467No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5730.1308210217738235No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5610.12808131451154445No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA5390.12305851786403288No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5320.12146035529437013No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5140.1173507944009516No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4900.11187137987639353No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4880.11141476199934704No Hit
GCGCTGGCTCGCCTGGACGCCGAACAGCGTG4810.10981659942968428No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC4800.109588290491161No Hit
TCTAAAGGCGACGCGAAGGGCTTTCGCGAAC4790.10935998155263778No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4710.10753351004445175No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4540.10365225808955647No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4530.10342394915103321No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC4490.1025107133969402No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4490.1025107133969402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)