FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007926320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400840
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60061.4983534577387487No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG54891.3693743139407244No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44141.1011875062369025No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG40251.0041413032631474No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG38520.9609819379303463No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG33240.8292585570302364No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24960.6226923460732462No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG21150.5276419519010079No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17150.4278515118251672No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG17080.42610517912383994No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA12920.32232312144496555No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12790.31907993214250074No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG10510.2621993812992715No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC9670.24124338888334498No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA9360.2335096297774673No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8070.20132721285300867No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7190.17937331603632373No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6960.17363536573196287No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6740.16814689152779164No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6730.16789741542760203No Hit
ACGGCAGCGACTTCCAAAGCCGTTGCTGCCA6180.15417622991717395No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5930.1479393274124339No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5740.14319928150883146No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA5550.13845923560522902No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5520.1377108073046602No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5210.12997704819878256No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5180.12922861989821374No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5040.12573595449555933No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG5020.12523700229518014No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4970.12398962179423212No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG4820.12024748029138808No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4720.11775271928949206No Hit
GCGCTGGCTCGCCTGGACGCCGAACAGCGTG4710.11750324318930247No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC4650.11600638658816485No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4580.11426005388683765No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4430.11051791238399361No Hit
GTTCAGATGCGTAAATCCATGGAAGAACAGA4300.10727472308152879No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4220.10527891428001197No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG4100.10228520107773675No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC4010.10003991617603034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)