FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448824
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG64281.4321872270645064No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG47231.0523055808067303No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG41260.9192913034953568No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG40110.8936687877653602No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28220.628754255565656No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22530.501978503823325No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19750.4400388571021157No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG19560.4358055718945511No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG18250.40661818441081576No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG17820.39703759157264323No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14680.327076983405522No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12970.2889774165374401No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG12540.27939682369926744No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT10250.22837459672388288No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT9370.20876780207832024No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8900.1982959913017129No Hit
ATCGCCGGTCGGGGTGCCGGTGGTCGGCTTCACCTGGACCAGCA7700.171559453148673No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7190.160196424433631No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7060.15729996613371836No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6920.154180703349197No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6840.15239826747232768No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA6820.15195265850311035No Hit
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA6430.14326328360337237No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6370.14192645669572038No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG5890.1312318414345044No Hit
TTCTTCGTCAGAGTCCTTCAGGTGACGGTAACCGTTTTTGCGGT5600.12477051138085307No Hit
GACTAAATTTAAATCCCTGTTTCACATGGTATCGCCCGGAACTA5490.12231966205015773No Hit
ACCGACATTTAAATCCGCGGCTCACATAGTGTCGCCCGGTACTA5470.1218740530809404No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5350.11920039926563641No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5250.11697235441954976No Hit
TTTGGCAACAACCTGTACTGGCGGCACCGGCGGCGGGCTCAGGT5220.11630394096572376No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5050.11251626472737644No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5030.11207065575815911No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG4960.11051102436589844No Hit
CAGTTTGCGGCTCGGAGTGTTCTGCATCAGTTCACGGAAACCCT4960.11051102436589844No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG4810.10716895709676844No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA4750.10583213018911646No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG4740.10560932570450779No Hit
AGTGATAACGTAACGGGTTTCGATCATGTCTTCCGGCTGCACGG4670.10404969431224712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)