FastQCFastQC Report
Sun 21 May 2023
EGAF00007926332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416916
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42841.027545117001986No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA32180.7718581201009317No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT27640.6629632827715894No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT27230.6531291675061642No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27170.6516900286868338No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23970.5749359583225397No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17210.4127929846779687No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13180.3161308273129359No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12790.3067764249872876No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12140.2911857544445404No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11170.2679196768653638No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11100.26624068157614483No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT10910.2616834086482649No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10250.2458528816356292No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9120.21874910053823793No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8790.2108338370319201No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7530.18061192182597935No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7440.17845321359698355No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC7370.17677421830776463No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7280.17461551007876885No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6540.15686613130702587No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6440.1544675666081417No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6220.14919072427059646No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6150.14751172898137754No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6030.14463345134271652No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6010.14415373840293969No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5960.1429544560534976No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5930.14223488664383233No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5770.1383971831256176No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5710.1369580443062871No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5560.13336019725796083No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5510.13216091490851875No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4980.11944852200443255No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4820.11561081848621783No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4680.11225282790777998No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4400.10553684675090426No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG4390.10529699028101584No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4260.1021788561724664No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4200.10073971735313589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)