FastQCFastQC Report
Wed 31 May 2023
EGAF00007926338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365555
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54691.496081301035412No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42141.1527677093734185No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29550.8083598911244546No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26350.7208217641668149No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26070.7131621780580214No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24260.6636484249976063No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23960.6554417255953277No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG19640.5372652542025139No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18920.5175691756370451No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17200.4705174323973137No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG16520.45191558041881524No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16120.44097331454911026No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15640.4278425955054643No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG15610.42702192556523644No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13920.38079085226573295No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13580.3714899262764837No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG13520.36984858639602797No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT13150.3597269904665508No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12840.3512467344175295No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12600.3446813748957065No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12240.33483333561297207No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA11810.3230703998030392No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11720.32060838998235563No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10980.3003651981234014No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10540.28832870566672597No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10400.2844989126123292No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9990.2732830900958816No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG9870.2700004103349701No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9310.25468123811738314No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8560.23416448961168634No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8360.22869335667683385No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC8300.22705201679637813No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8110.22185444050826825No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7820.21392129775273214No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7300.1996963521221157No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7120.19477233248074843No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7100.19422521918726318No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7020.1920367660133222No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG6680.182735840024073No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6540.17890604696967624No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6450.17644403714899262No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6220.17015223427391227No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5930.16221909151837616No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC5630.15401239211609744No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5620.15373883546935482No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5580.15264460888238432No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5540.15155038229541382No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5540.15155038229541382No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5310.14525857942033346No Hit
GCTTCTGAACCGGAAGACAAATCTCCGCGTG5200.1422494563061646No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5200.1422494563061646No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5190.14197589965942198No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4990.1365047667245695No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC4990.1365047667245695No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4940.13513698349085637No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4890.13376920025714326No Hit
GACGGTACCTCTAACGCCCTGTACCGTACCA4870.133222086963658No Hit
CTGGATGCTCACGCAATTAAGCCGGCCATCG4840.13240141702343014No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4550.12446827426789403No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4260.11653513151235792No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4140.11325245175144642No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG4090.1118846685177333No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4010.10969621534379231No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4010.10969621534379231No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG3840.1050457523491677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)