FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410124
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48881.191834664638012No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT32670.7965883488896042No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30260.7378256332231228No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29540.7202699671318918No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28050.6839394914708722No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26200.6388311827642372No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG22080.5383737601310823No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG21100.5144785479513513No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20050.4888765349016395No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG18410.4488886288049468No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18410.4488886288049468No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18390.4484009714135237No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG18190.44352439749929284No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG16290.3971969453141001No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15520.3784221357443115No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG15390.37525236270006146No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15330.3737893905257922No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15130.3689128166115614No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT14290.34843120617179196No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14040.34233548877900344No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13390.32648662355775326No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12950.3157581609464455No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA12630.30795564268367615No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12580.3067364992051185No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12180.2969833513766568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12000.29259443485384906No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11570.28210980093825283No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG11230.27381962528406045No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10140.24724229745150247No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC9810.23919595049302161No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9790.23870829310159852No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9680.23602617744877158No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9640.23505086266592543No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9090.22164028440179068No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8830.21530073831329063No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8820.21505690961757906No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG8140.1984765583091943No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8050.19628210004779043No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8000.19506295656923273No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7790.18994255395929036No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC7350.17921409134798255No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6970.169948600910944No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC6790.16555968438813626No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6760.16482819830100165No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC6730.164096712213867No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6610.16117076786532852No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC6600.16092693916961698No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6510.15873248090821312No Hit
CTGGATGCTCACGCAATTAAGCCGGCCATCG6440.15702568003823233No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6340.15458739308111694No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6270.15288059221113615No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6110.1489793330797515No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6080.14824784699261687No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG6080.14824784699261687No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5930.14459041655694374No Hit
GACGGTACCTCTAACGCCCTGTACCGTACCA5880.14337127307838604No Hit
GCTTCTGAACCGGAAGACAAATCTCCGCGTG5730.13971384264271294No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5700.1389823565555783No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5610.13678789829417443No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG4970.12118286176863582No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4610.11240502872302036No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4530.11045439915732802No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4490.10947908437448185No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4340.10582165393880874No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC4290.10460251046025104No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4290.10460251046025104No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4220.10289570959027025No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG4190.10216422350313563No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4160.10143273741600102No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4130.1007012513288664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)