FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007926342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382491
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49581.29623965008327No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC44221.1561056338580515No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31260.8172741319403595No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27990.7317819242805713No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26770.6998857489457267No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25290.6611920280477188No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24290.6350476220355511No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG20680.5406663163316261No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19890.5200122355820137No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17840.46641620325707006No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16890.4415790175455109No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG16830.44001035318478077No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG16810.43948746506453745No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16390.42850681453942707No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG14770.38615287679971555No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13920.3639301316893731No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13610.35582536582560115No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13610.35582536582560115No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT13570.35477958958511446No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13320.34824348808207256No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12530.3275894073324601No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12300.3215761939496616No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12000.3137328721460113No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA11790.3082425468834561No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11020.288111354254087No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10530.2753005953081249No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10300.26928738192532636No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG9810.2564766229793642No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9600.250986297716809No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9270.2423586437327937No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC8700.22745633230585818No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8330.21778290208135614No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8320.21752145802123446No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8090.21150824463843595No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7790.20366492283478566No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7240.18928549952809348No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7110.1858867267465117No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7020.1835337302054166No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6950.18170362178456487No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC6950.18170362178456487No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG6780.17725907276249636No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6430.1681085306582377No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC6330.16549409005702095No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6300.16470975787665593No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5970.15608210389264063No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5970.15608210389264063No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5930.15503632765215392No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5690.14876167020923367No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5510.1440556771270435No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5470.1430099008865568No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC5370.14039546028534006No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5360.14013401622521837No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5350.13987257216509671No Hit
GACGGTACCTCTAACGCCCTGTACCGTACCA5330.13934968404485334No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5320.13908823998473166No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5140.1343822469025415No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5080.13281358254181144No Hit
CTGGATGCTCACGCAATTAAGCCGGCCATCG4990.13046058600071636No Hit
GCTTCTGAACCGGAAGACAAATCTCCGCGTG4680.12235582013694439No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG4620.12078715577621435No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4580.11974137953572764No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4420.11555827457378082No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG4320.11294383397256406No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4290.11215950179219902No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4210.11006794931122561No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG3990.10431617998854874No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG3940.10300895968794037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)