FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007926363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544529
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30090.5525876491426536No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28730.5276119361870534No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC27680.5083292166257444No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT27380.5028198681796562No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27140.4984123894227856No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG21880.40181514666803786No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19610.36012774342597No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18370.3373557698488051No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14200.26077582644817815No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG13690.2514099340898281No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12880.23653469328538979No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12130.22276132217016906No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11620.21339542981181903No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11580.21266085001900725No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA11480.21082440053697785No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11450.21027346569236902No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10250.18823607190801592No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10030.1841958830475512No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9750.1790538244978688No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8590.15775101050632748No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8130.14930334288899214No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8030.1474668934069627No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC7970.14636502371774507No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7840.1439776393911068No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7780.14287576970188914No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7600.1395701606342362No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7390.1357136167219744No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7250.13314258744713323No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA6930.12726594910463906No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6840.12561314457081257No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT6730.12359305014058022No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT6680.12267482539956549No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6670.12249118045136256No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT6480.11900192643550665No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6040.11092154871457718No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5980.10981967902535952No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5900.108350519439736No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5820.10688135985411244No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC5770.10596313511309775No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC5670.10412668563106833No Hit
GTACTCCAGTCGGTGTTTCCGCCCTCTACGCGGCGAAGCGACCG5520.10137201140802418No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5510.10118836645982125No Hit
GAAGCCACCTTTGGAGCGGGTTTTAGAATCACGCTTACGGCGCA5450.10008649677060359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)