FastQCFastQC Report
Wed 31 May 2023
EGAF00007926364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544529
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61351.1266617572250515No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA59471.092136506962898No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29260.5373451184418093No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27230.500065193956612No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25290.4644380740052413No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23390.42954553384668215No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20450.3755539190750171No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20240.3716973751627553No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18500.3397431541754434No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA16280.29897397567439016No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT13740.2523281588308428No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA13490.24773703512576928No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13220.2427786215242898No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13000.23873843266382505No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG12390.22753609082344556No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11790.21651739393126904No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11740.21559916919025435No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA10390.1908071011828571No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC9930.18235943356552176No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9660.1774010199640423No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT9650.17721737501583937No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8950.1643622286416334No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8680.15940381504015397No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8650.15885288019554514No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8230.15113979237102157No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8200.15058885752641274No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7890.14489586413212152No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7250.13314258744713323No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7070.12983697837948024No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG6630.12175660065855078No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6570.12065473096933314No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6060.11128883861098307No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5860.10761593964692422No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG5710.10486126542388009No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5610.10302481594185067No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5480.10063743161521242No Hit
GGCGCACCGTCCGGCGGCCCGAGCGACGTTA5470.10045378666700946No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5460.10027014171880652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)