FastQCFastQC Report
Wed 31 May 2023
EGAF00007926377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504521
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG57551.1406859179300761No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT46630.9242429948406508No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT30950.6134531565583989No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT19350.38353210272714117No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA18830.37322529686573996No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT17720.3512242305077489No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG15230.3018704870560393No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA13880.27511243337740154No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT11360.22516406651061105No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT11180.22159632602012602No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG10510.2083164030833206No Hit
CTGTGCGCGGTCGCCGCTACCCGGAGACACCAGACCGCCCGGCA8440.1672873874427427No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG8270.16391785475728463No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7300.14469169766967085No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG6920.1371598010786469No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6910.13696159327361992No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6800.1347813074183235No Hit
TTTAACGTTAGAGGTGAATACGCCACCGAAGCGTGGACCCAGAC6610.13101535912281154No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6430.1274476186323265No Hit
CAGGGTCGGAATCTGATGACGGGTTTTGCATGCAACACCGTTCT6410.1270512030222726No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6340.12566374838708397No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA6130.12150138448151812No Hit
AGATTTCATAGCGAATGGCAGGGTCGCAATGTCTGCAATGGTCG6120.12130317667649115No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT6020.1193210986262217No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC5980.11852826740611391No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA5750.11396948789049416No Hit
GGATGGCGGAGTCAGGCCTTTTTCCTTGGCGTAAGCCTGTGCCG5720.11337486447541331No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5500.10901429276482048No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT5430.10762683812963188No Hit
GTTCAGGCCACGGATCTGACCGCGCATTTTTTCGCTAATGGACA5380.10663579910449714No Hit
CACAGTCAGAGCAGTGGTAGATTCGAAGTCTTTAACATCCGCGG5190.10286985080898517No Hit
ACGCACCAGACCCTGTTTCAGCACCTGGTCAATGCGCTCCTGCA5050.10009494153860791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)