FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530229
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG59121.1149899383096737No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT48140.9079096013232018No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA33410.6301051055298749No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG21150.39888425567066305No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA20100.3790814912047436No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC18890.3562611626297317No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT16790.3166556336978928No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG15150.28572560157969484No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT12430.23442701172512256No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT12080.22782609023648273No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC12060.22744889472284618No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA11690.2204707777205698No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9660.18218543308645888No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG9550.18011085776145777No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT9450.17822488019327498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9140.17237834973190827No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8520.1606852888091749No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8280.15615894264553618No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA8040.15163259648189745No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG7900.1489922278864415No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7810.14729484807507698No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG7530.14201411088416513No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA7270.13711056920688985No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT6860.12937806117734035No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG6750.12730348585233928No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC6540.12334293295915538No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6290.11862798903869837No Hit
GGTCGTAAAGTACGCGGTGTCATTCCGACTG6210.11711920698415214No Hit
AAACTGGCAGGCAAATACACTACCGAGGACG6170.116364815956879No Hit
ATGGGTGTTGACATGGTCGAAATCGACCTGA6160.11617621820006073No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6160.11617621820006073No Hit
GTATATAGCAACGTTGAAGTCACCAACGGCA6000.11315865409096824No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA5850.11032968773869403No Hit
GGTGTTCCGGCGTCTACCTGGGCCGCAATCA5700.10750072138641983No Hit
GAAGTTCACACCATGGTTGTACAGCACAACA5700.10750072138641983No Hit
CGCACCGGCGTTTCCGCCTCTACCTGGAACA5440.10259717970914455No Hit
CTGGACAGCCTGCGCATGGAGATGGAACGTC5420.10221998419550797No Hit
GGTTACCAGAAACTGCGTCATAACATCGACA5400.10184278868187141No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5350.10089979989778002No Hit
CGTAGCCTGCGCCAGGCAAAAAACATGCTGG5310.10014540887050691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)