FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007926399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549104
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT34000.6191905358547742No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26760.4873393746904047No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25920.4720417261575221No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA24940.45419446953582565No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22000.4006526996707363No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21620.3937323348582418No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20360.37078586205891784No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18950.34510766630729334No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17260.3143302543780413No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC14790.26934788309682683No Hit
ACGACGGGAGGAGCGAGAGTTCACAGACGGACGACGACCTGGGC13580.24731198461493634No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13040.23747778198665465No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11350.20670037005740258No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10850.1975946268830677No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10570.19249541070544013No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10260.18684984993735249No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10200.1857571607564323No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8830.16080742445875462No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8720.15880416096040095No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8700.15843993123342753No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8680.15807570150645414No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7790.141867478656138No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7670.13968210029429762No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7540.13731460706897053No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7230.13166904630088289No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6990.12729828957720213No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6690.12183484367260118No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6460.1176462018124071No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6150.11200064104431948No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6060.1103616072729392No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6010.1094510329555057No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5870.10690142486669192No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5850.10653719513971853No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5810.10580873568577173No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5750.10471604650485154No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5620.10234855327952445No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT5550.10107374923511757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)