FastQCFastQC Report
Wed 31 May 2023
EGAF00007926414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489217
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA39580.8090479276067675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39420.805777395307195No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25370.5185837777509775No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20470.41842372607656725No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18940.38714926096190444No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17820.36425553486489637No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17500.3577144702657512No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14940.3053859534725899No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14470.2957787648425954No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14250.2912817829306831No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12890.26348225838431616No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12570.256941193785171No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12550.25653237724772443No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12020.24569873900539022No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11140.22771081135774104No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10440.213402232547111No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10350.2115625581286014No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9890.20215977776733024No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG9630.19684516278052477No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8760.17906164340159889No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC8690.17763078552053588No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7940.1623001653662894No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7850.1604604909477798No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7780.1590296330667168No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7710.1575987751856538No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7580.1549414676922511No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7270.1486048113618292No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG7250.14819599482438262No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7220.1475827700182128No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7070.14451664598736347No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7050.1441078294499169No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6610.1351138656260923No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6070.12407581911503483No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6000.12264496123397184No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5860.11978324547184582No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5830.11917002066567596No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5680.11610389663482668No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5630.11508185529121025No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5460.11160691472291437No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5340.10915401549823495No Hit
CGTGATCTGGAAGCGGTACGCCAGGCCAAAA5230.10690552454227878No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5130.10486144185504592No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5000.1022041343616432No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4990.10199972609291992No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4930.1007732764805802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)