FastQCFastQC Report
Sun 21 May 2023
EGAF00007926439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553385
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG59031.066707626697507No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG58421.0556845595742568No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA38150.6893934602491936No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA34500.6234357635281044No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG27140.490436133975442No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG24790.4479702196481654No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17750.32075318268474934No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG16730.3023211688065271No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15850.2864190391861001No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15040.2717818516945707No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14890.2690712614183616No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG14860.2685291433631197No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG14800.26744490725263603No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14700.26563784706849664No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13600.24576018504296285No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13550.24485665495089315No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12880.23274935171715894No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12170.21991922440976897No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11880.2146787498757646No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11380.20564344895506745No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA10980.19841520821850972No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9920.17926037026663172No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9740.17600766193518075No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG9320.1684180091617951No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9290.1678758911065533No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9260.16733377305131147No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT9190.16606883092241387No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9140.16516530083034417No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9030.16317753462779078No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8920.1611897684252374No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8540.15432293972550756No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8340.15070881935722868No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8010.14474552074956856No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7980.14420340269432674No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7800.14095069436287577No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT7710.13932434019715026No Hit
TTTGACGATGTACTTACGGTTGTTGTGAATATAGATACCTTTGT7700.13914363417873632No Hit
CGGGGTGTTACCACCGATCGTCAGAGTATCGGAGACTTTCGCAG7680.13878222214190844No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA7410.13390315964473198No Hit
GCGACGACCGGCCGCAAATTCTTTAAATTCACGCAGTGCCAGCG7350.13281892353424832No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7140.1290240971475555No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7130.12884339112914156No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT6960.12577138881610453No Hit
GCCCAGGGTGATAGACGTCAGACCGGTCAGAGTGTCCTGCAGGC6650.12016950224527229No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6460.11673608789540735No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6440.11637467585857947No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6240.1127605554903006No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6150.11113420132457513No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6110.11041137725091935No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5920.10697796290105442No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5810.10499019669850106No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5780.10444807864325922No Hit
GTTCAGGTCGTTTGCCAGAGCAAAGGTAATGTTTTTACCGTCAC5680.10264101845911977No Hit
GATAACGGTTTTCGCATCGTTACCACCGTTAGTGGTACCGCCCA5540.10011113420132457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)