FastQCFastQC Report
Sun 21 May 2023
EGAF00007926440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553385
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT83001.4998599528357293No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG58341.054238911426945No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG57101.0318313651436162No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT26940.48682201360716315No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26670.48194295110998675No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG26570.4801358909258473No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17880.3231023609241306No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17650.3189461225006099No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16960.3064774072300478No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG16870.3048510530643223No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16560.29924916649349004No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG16300.29455081001472755No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16130.29147880770169055No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15900.2873225692781698No Hit
TCCTACACCAACGGCAAACTGACTATCACCC14750.2665413771605663No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14630.264372904939599No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14380.2598552544792504No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13760.24865148133758594No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA13580.245398773006135No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11920.21540157394942036No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG11270.20365568275251406No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10270.18558508091111975No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10230.18486225683746396No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10220.18468155081905002No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10180.18395872674539426No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9690.17510413184311102No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG9120.1648038887935163No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9120.1648038887935163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.16191259249889317No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA8920.1611897684252374No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8470.15305799759660996No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA8410.1519737614861263No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8320.1503474073204008No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC8290.149805289265159No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8260.14926317120991714No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8090.1461911688968801No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7960.14384199065749886No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7630.1378786920498387No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7580.136975161957769No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG7530.1360716318656993No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7520.13589092584728535No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7440.13444527769997378No Hit
ATCCTGGCATTCACCCTGTACACTGAAGCTA7330.1324575114974204No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG7200.13010833325803917No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7110.12848197909231365No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6860.12396432863196509No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6790.1226993865030675No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT6560.11854314807954679No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6440.11637467585857947No Hit
AAAGCGATCAACGATGCTGCAACTTTCGTTA6190.11185702539823089No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6180.11167631937981694No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6130.11077278928774723No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG5690.10282172447753372No Hit
ATGGCATCCACCACCCCGATCACCATGGAAG5680.10264101845911977No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5570.1006532522565664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)