FastQCFastQC Report
Wed 31 May 2023
EGAF00007926441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550993
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG57291.0397591257965164No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG56641.0279622427145172No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA39430.7156170768049684No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA35750.6488285695099575No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG27800.5045436148916592No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG25630.4651601744486772No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17480.31724540965130227No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16760.30417809300662624No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG16700.3030891499529032No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15070.2735061969934282No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14810.2687874437606285No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG14790.26842446274272086No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG14560.26425018103678266No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG14550.26406869052782883No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT12750.23140039891613867No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12740.23121890840718484No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12120.21996649685204714No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11920.21633668667297043No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11790.2139773100565706No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11530.20925855682377092No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA10530.19110950592838746No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9750.1769532462299884No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9590.17404939808672706No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9530.17296045503300406No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9370.1700566068897427No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG9200.16697126823752753No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8980.16297847704054316No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8790.1595301573704203No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8700.15789674278983581No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT8390.15227053701226692No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8350.1515445749764516No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8330.15118159395854394No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8270.15009265090482093No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8080.14664433123469808No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7740.1404736539302677No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7640.13865874884072937No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7460.13539191967956035No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT7370.13375850509897586No Hit
CGGGGTGTTACCACCGATCGTCAGAGTATCGGAGACTTTCGCAG7090.12867677084826848No Hit
TTTGACGATGTACTTACGGTTGTTGTGAATATAGATACCTTTGT7050.12795080881245316No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6900.12522845117814563No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT6900.12522845117814563No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA6850.12432099863337648No Hit
GCGACGACCGGCCGCAAATTCTTTAAATTCACGCAGTGCCAGCG6770.1228690745617458No Hit
GCCCAGGGTGATAGACGTCAGACCGGTCAGAGTGTCCTGCAGGC6630.12032820743639212No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6490.11778734031103844No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6140.11143517249765424No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6040.10962026740811588No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6010.10907579588125438No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5940.10780536231857756No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5840.10599045722903921No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5810.10544598570217771No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5730.10399406163054703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)