FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550993
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT80981.4697101415081497No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG56481.025058394571256No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG55671.0103576633459952No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG27760.5038176528558439No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT27640.5016397667483979No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26760.4856686019604605No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17870.32432353950050186No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17530.3181528621960715No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG16870.30617448860511837No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16850.30581150758721076No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16700.3030891499529032No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG15950.2894773617813656No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15880.2882069282186888No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15700.2849400990575198No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14860.2696948963053977No Hit
TCCTACACCAACGGCAAACTGACTATCACCC14580.2646131620546904No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13990.25390522202641413No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13730.24918646879361445No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA12570.22813356975496965No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11940.21669966769087812No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG11020.20000254086712535No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10720.19455782559851031No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10190.1849388286239571No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10170.18457584760604945No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10040.1822164709896496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9790.17767920826580375No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9250.16787872078229668No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9170.16642679671066601No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG8850.16061910042414332No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8790.1595301573704203No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8460.15354097057494379No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA8240.14954817937795942No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8220.14918519836005176No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7970.1446479356362059No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7920.14374048309143672No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7880.1430145210556214No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC7850.1424700495287599No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7780.14119961596608305No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA7770.14101812545712922No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7770.14101812545712922No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG7040.1277693183034993No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7030.1275878277945455No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6940.12595441321396098No Hit
ATCCTGGCATTCACCCTGTACACTGAAGCTA6850.12432099863337648No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT6820.12377652710651497No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6670.12105416947220744No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6530.11851330234685378No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6440.11687988776626927No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6420.11651690674836158No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6380.11579094471254626No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5940.10780536231857756No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5920.10744238130066988No Hit
ATGGCATCCACCACCCCGATCACCATGGAAG5780.10490151417531621No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG5690.10326809959473171No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5660.10272362806787018No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5550.10072723246937801No Hit
AAAGCGATCAACGATGCTGCAACTTTCGTTA5540.10054574196042418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)