FastQCFastQC Report
Wed 31 May 2023
EGAF00007926443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580376
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA34230.5897900671288958No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30950.5332749803575613No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT22370.3854397838642535No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21900.37734158545494645No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16620.2863660799206032No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16110.27757867313603596No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG15480.2667236412256882No Hit
CAGGCCGGTGCGCAGGTTGGTGAATTTAGCAATCGGCAGGCCAC14760.254317890471005No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA13210.22761106592967317No Hit
TTCGTCACCGGTGCTCGGCAGCTTACGCTTGTTGTTAGTGGATT12790.22037437798944132No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12610.21727294030077055No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12250.21107006492342897No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12020.2071071167656829No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11870.20452258535845727No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11410.19659668904296523No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11100.1912553241346989No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10420.17953878175527588No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9690.16696072890677766No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG9450.16282547865521663No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9320.16058555143562103No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8890.15317656140157415No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC7820.13474023736336443No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7500.12922657036128302No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7360.12681434104787243No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7180.12371290335920161No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7150.12319599707775648No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7060.12164527823342108No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6680.11509779866844942No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6420.11061794422925826No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6080.10475967303954677No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6000.10338125628902642No Hit
CGCATCAATAACAGCTACAACGGTACCAGCGCCTTTACCTGCCT5950.1025197458199512No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5950.1025197458199512No Hit
AATCAGACGCGCGGTGGTTACGTTCGTAGATTTCGCCAGCTTTG5900.10165823535087598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)