FastQCFastQC Report
Wed 31 May 2023
EGAF00007926444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580376
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT71681.2350614084662357No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31810.5480929604256551No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22090.3806153252374323No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17860.30773153955366866No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17610.30342398720829256No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG16170.2786124856989262No Hit
GAACGTACCACTGACGGTAAACTGGATCCGC15950.27482183963499524No Hit
TCCCAGGAAATCACCTTCGTTTACGAGAAAA13500.23260782665030943No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13470.2320909203688643No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13390.23071250361834397No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13350.2300232952430838No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13140.2264049512729679No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12840.22123588845851652No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12450.21451610679972982No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12000.20676251257805284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10840.18677546969550773No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10390.17902187547383075No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9970.17178518753359892No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9390.16179166609232634No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8670.14938591533764317No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8110.13973699808400072No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7950.13698016458296003No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7940.13680786248914498No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG7880.1357740499262547No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7850.13525714364480956No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7610.13112189339324853No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7550.13008808083035825No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6590.11354707982411402No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6570.11320247563648395No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6540.1126855693550388No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6120.10544888141480696No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC6050.10424276675810164No Hit
GTGGGTACCTTCGAAAAAGTCTTTAACGACG6010.10355355838284146No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG5880.1013136311632459No Hit
GCTGGTCACCTGATCGGTAAAGGCGCGAAAG5810.1001075165065406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)