FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541383
Sequences flagged as poor quality0
Sequence length31
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55881.0321713094057257No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23800.43961483829377723No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17260.31881311382145355No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG17150.31678128053522187No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17070.31530358359978056No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16030.29609352343904405No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA13500.24936135785571398No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC11540.2131577829374029No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9640.1780624807206728No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9190.16975043545881566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8780.16217723866467917No Hit
GAAAAACAGCGTAAACAAAAAGATCTGATTA8450.15608173880598394No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA8390.15497346610440296No Hit
GTTCCGGAAGAAATCATCCCGGTTACCAAAC8390.15497346610440296No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8260.15257220858431092No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8100.1496168147134284No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7670.14167419368543158No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG7330.13539398170980618No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG7170.13243858783892365No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7130.131699739371203No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6780.12523481527864747No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6730.12431125469399666No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG6640.12264884564162525No Hit
GGTCGTAAAGTACGCGGTGTCATTCCGACTG6140.11341323979511733No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG5650.10436234606553955No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5640.10417763394860939No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5450.10066810372693638No Hit
ACCGCCACCGTTAAAGCATATGGCGGCGAAG5430.10029867949307607No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5420.1001139673761459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)