FastQCFastQC Report
Wed 31 May 2023
EGAF00007926455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517783
Sequences flagged as poor quality0
Sequence length44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25700.4963469252563332No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT22510.434738104572765No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22290.4304892203876914No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16080.310554807709021No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13630.263237688375246No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13630.263237688375246No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12780.24682154493291592No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT10580.20433270308217924No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8500.1641614344233009No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG8010.1546980105565459No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7880.152187306265366No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7620.1471658976830062No Hit
GCGGAAGGCATCGGAGTCATAGCGACGCTTGTCCTGTGCGATGT7540.14562084888843396No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7520.1452345866897909No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC7450.1438826689945402No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA7390.142723882398611No Hit
GTCCAGGTGCATGGTGTCACGGATTTTCTGGTACACGGCAGAAG7250.14002004700810958No Hit
AAACAGACGGCCCTGCAGCAGCGGATCGTCGGTGAAGTCCACAC7070.13654368722032204No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG7040.13596429392235745No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6870.13268106523389142No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6800.1313291475386407No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG6080.11742370838749051No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5970.11529926629495368No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT5880.1135610864010599No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5780.1116297754078446No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT5390.10409766253430491No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5280.10197322044176807No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5240.10120069604448195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)