FastQCFastQC Report
Wed 31 May 2023
EGAF00007926457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524894
Sequences flagged as poor quality0
Sequence length44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25910.4936234744538897No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22970.43761216550389215No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT22830.43494496031579705No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16180.3082527138812789No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13830.26348176965253933No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13150.25052677302464876No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12260.23357096861461552No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT10870.20708943138995684No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8690.16555723631818997No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8290.1579366500664896No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8000.15241172503400688No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7880.15012554915849677No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC7350.14002827237499382No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG7340.1398377577187013No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA7300.13907569909353126No Hit
GCGGAAGGCATCGGAGTCATAGCGACGCTTGTCCTGTGCGATGT7200.1371705525306062No Hit
GTCCAGGTGCATGGTGTCACGGATTTTCTGGTACACGGCAGAAG7200.1371705525306062No Hit
AAACAGACGGCCCTGCAGCAGCGGATCGTCGGTGAAGTCCACAC7200.1371705525306062No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7160.13640849390543613No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7120.13564643528026613No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6520.12421555590271559No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5990.11411827911921264No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT5870.11183210324370253No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5860.11164158858741004No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG5720.10897438339931491No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5660.10783129546155985No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT5550.10573563424234227No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5480.10440203164829469No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5450.10383048767941719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)