FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549189
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA29710.5409795170697156No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA26460.4818013470772357No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24960.45448834554224504No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24590.4477511384969473No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG20370.3709105608451735No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20280.3692717807530741No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18790.3421408658949833No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT17300.3150099510368926No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT15490.2820522625180038No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG15140.275679228826506No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14060.25601386772131274No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13240.2410827602155178No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12790.23288885975502058No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12180.2217815724641244No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT12170.22159948578722444No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT10530.19173727077563463No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10260.1868209304993363No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9960.18135833019233816No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9450.17207190967044134No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9330.16988686954764207No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT9120.16606304933274338No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8990.16369592253304416No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8980.16351383585614424No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8900.16205714244094474No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8830.16078253570264517No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8630.15714080216464643No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8620.15695871548774648No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8530.15531993539564704No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8140.14821855499654946No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7970.14512308148925052No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7680.1398425678591523No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7400.13474414090595405No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT7210.13128449404485523No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6970.12691441379925672No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG6940.1263681537685569No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6750.1229085069074581No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6410.11671755989286019No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6150.11198330629346182No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5690.10360731915606466No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5650.10287897244846492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)