FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549189
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63251.1516982313921074No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35010.6374854558266826No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26130.4757924867395378No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20360.3707284741682736No Hit
ATGTCCAACATGGACATCGATGGTATTAACA20340.3703643008144737No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19650.357800320108378No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18290.33303653204998646No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15350.2795030490414047No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15190.27658966221100567No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15050.2740404487344065No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14480.2636615081511101No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14470.26347942147421016No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT14250.2594735145824115No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12340.2246949592945234No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10960.19956699788233195No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10740.1955610909905333No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10700.19483274428293357No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10270.18700301717623624No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9850.17935537674643884No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9720.17698824994673965No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9700.17662407659293977No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9700.17662407659293977No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9380.17079730293214176No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9100.1656988759789435No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8860.161328795733345No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8730.1589616689336458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8710.15859749557984593No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8660.15768706219534623No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8660.15768706219534623No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8590.15641245545704666No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8420.15331698194974772No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7930.14439473478165077No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT7670.13966048118225238No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7520.13692918102875332No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7490.1363829209980535No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7380.13437996755215417No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC7220.13146658072175518No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6940.1263681537685569No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6580.11981303340015916No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6390.11635338653906031No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6080.11070869955516224No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5890.10724905269406343No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5780.10524609924816412No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5560.10124019235636549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)