FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520163
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA29890.5746275686659759No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA26260.5048417515278865No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23330.4485132544990705No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT22030.42352108858184845No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19720.379111932221246No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG19530.37545923104872897No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18160.34912133312057947No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15870.3050966716202421No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT15080.28990912463977636No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13550.26049526782950727No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG13470.2589572883884475No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13150.2528053706242082No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11740.22569848297552883No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11470.22050780236195192No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11320.21762409090996473No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9960.1914784404119478No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9930.19090169812155036No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9550.1835962957765162No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9360.17994359460399914No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8740.16802425393578552No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8720.16763975907552056No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8690.16706301678512311No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8540.16417930533313596No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8500.16341031561260605No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8220.15802738756889667No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7990.1536056966758497No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7900.15187546980465738No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7680.14764602634174287No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7650.14706928405134545No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7430.14283984058843094No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7340.14110961371723865No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7100.13649567539405916No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7010.13476544852286687No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6900.1326507267914096No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG6600.12688330388743527No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT6420.1234228501450507No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6300.12111588098346095No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5580.10727406601392256No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5480.1053515917125978No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5430.10439035456193538No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5340.1026601276907431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)