FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520163
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61191.176362024980631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41060.7893679481239535No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32780.6301870759742619No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22510.4327489652282073No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19430.3735367567474042No Hit
ATGTCCAACATGGACATCGATGGTATTAACA19190.3689228184242247No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18690.35931044691760083No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16400.31528578541726343No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15570.29932924871626776No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14550.27972001084275505No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13550.26049526782950727No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT13140.2526131231940757No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13120.25222862833381077No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13040.25069064889275094No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11290.21704734861956732No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10620.20416677080069132No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10520.20224429649936654No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9510.1828273060559863No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9460.18186606890532392No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9170.17629089343148205No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9040.17379167683975985No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8960.17225369739870003No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8910.17129246024803763No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8640.16610177963446074No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8630.16590953220432827No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8480.1630258207523411No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8400.16148784131128127No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7840.15072198522386251No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7840.15072198522386251No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7610.14630029433081554No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7560.14533905718015314No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7550.14514680975002067No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7190.13822590226525147No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6890.13245847936127714No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC6680.12842128332849512No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6630.12746004617783271No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6500.1249608295861105No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6430.12361509757518316No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6420.1234228501450507No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6050.116309695230149No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5860.11265699405763194No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5650.1086197980248499No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5300.1018911379702132No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5230.10054540595928585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)