FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545529
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60161.1027828034806584No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35520.6511111233316652No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24900.4564376962544613No Hit
ATGTCCAACATGGACATCGATGGTATTAACA21830.4001620445475859No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20670.3788982803847275No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19750.36203391570383975No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18130.33233796920053743No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15770.28907720762782546No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15310.2806450252873816No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT14640.26836336840021335No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14310.2623141941125036No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14250.26121434424201095No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT14190.2601144943715183No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12520.2295020063094721No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11250.20622185071737706No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10960.20090590967666247No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10300.18880756110124303No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10270.18825763616599667No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10090.18495808655451865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9790.17945883720205524No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9370.1717598881086065No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9280.1701101133028675No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9100.16681056369138947No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8960.16424424732690654No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8750.16039477278018216No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8740.1602114644684334No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8690.1592949229096895No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8530.15636198992170902No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8470.15526214005121636No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8160.14957958238700417No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7960.14591341615202857No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7700.14114740004656032No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7660.1404141667995652No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT7400.13564815069409694No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7310.13399837588835792No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7160.1312487512121262No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC6740.12354980211867746No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6660.12208333562468722No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6620.1213501023776921No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6610.12116679406594334No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6600.12098348575419456No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5900.10815190393177998No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5730.10503566263205073No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5550.10173611302057269No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5460.10008633821483368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)