FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436348
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA79021.8109398920127973No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43010.9856811535746697No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT42050.9636803652130868No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA40560.92953330827688No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA36010.8252587384381274No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32610.7473392796575211No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27940.6403146112735706No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC26190.6002090074894351No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23010.5273313960416915No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC21820.5000595854684793No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC21570.49433021349931705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20480.46935015171376976No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16870.3866180204790672No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC16560.379513599237306No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16040.36759650554144857No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15310.3508667393914948No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14060.3222198795456837No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12550.28761447285194386No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11830.27111388158075667No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11650.26698873376295984No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11630.2665303840054269No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10960.2511756671280721No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9780.22413303143362637No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9780.22413303143362637No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9540.21863283434323064No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9410.21565356091926627No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8150.18677752619468863No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT8130.18631917643715568No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7060.16179746440914133No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7000.1604224151365424No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG6840.15675561707627858No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6630.1519429446221823No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6280.14392182386535518No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6040.13842162677495945No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5940.13612987798729453No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5850.13406730407839615No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5810.1331506045633302No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5570.12765040747293444No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5060.11596248865584351No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC5010.11481661426201105No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC4990.11435826450447809No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4840.11092064132298073No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4720.10817054277778286No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4680.10725384326271692No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4630.10610796886888446No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4560.10450374471751905No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4500.10312869544492011No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG4370.10014942202095575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)