FastQCFastQC Report
Sun 21 May 2023
EGAF00007926500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429499
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGTCCGGTGTACCCGAAACCGGTTC46961.0933669228566307No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46961.0933669228566307No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA42830.9972083753396399No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG26330.6130398440974252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20910.4868463023196794No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20440.4759033199145982No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17910.4169974784574586No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17610.4100125960712365No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17560.4088484490068662No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16990.3955771724730442No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16390.38160740770060003No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG16320.3799776018104815No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14120.3287551309781862No Hit
CGTGTTAACAGCAACGTGCTGTACGGTACCG13010.3029110661491645No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12670.29499486611144615No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12230.2847503719449871No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC11000.2561123541614765No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10710.24936030118812852No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG10420.24260824821478047No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10060.23422638935131396No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9910.23073394815820292No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9720.22631018931359562No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG8430.19627519505284063No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7760.18067562439027798No Hit
ATGGCAATTAAACACACCACTATCGACATCG7240.16856849492082637No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7240.16856849492082637No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7020.16344624783759684No Hit
CCGTACGATCCGTTCGACCGTTCCCACCTGA6830.1590224889929895No Hit
GAGAAACCGTACGATCCGTTCGACCGCAGCC6230.14505272422054533No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6140.14295725950467872No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5870.13667086535707884No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5660.13178144768672337No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5630.13108295944810117No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5440.12665920060349384No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5410.12596071236487164No Hit
TACATCCTGAAAAAAGGTGAAGAACCGTACG5310.12363241823613094No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5030.11711319467565698No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4960.1154833887855385No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4920.11455207113404223No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG4760.1108268005280571No Hit
ACCGACCTGATTAGCGAAAAATACTACATCC4730.1101283122894349No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4720.10989548287656083No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4600.10710152992207199No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4540.10570455344482757No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4430.10314342990321282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)