FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402054
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67391.6761430056659057No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC43201.0744825321971676No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT42701.0620463917782188No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA38940.9685266158277247No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG24930.6200659612887821No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18370.4569037989921752No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16940.4213364373939819No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16610.41312858471747577No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15800.3929820372387789No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15700.39049480915498913No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15190.3778099459276615No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG14700.36562252831709174No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12870.3201062543837395No Hit
CGTGTTAACAGCAACGTGCTGTACGGTACCG12350.30717266834803286No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12080.30045715252180055No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11360.28254911031851443No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC10360.25767682948061704No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9760.2427534609778786No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG9650.24001751008570987No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9400.2337994398762355No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8990.22360180473269758No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8950.2226069134991817No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG7220.1795778676496192No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6960.1731110746317659No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC6760.1681366184641864No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6700.16664428161391256No Hit
CCGTACGATCCGTTCGACCGTTCCCACCTGA6570.16341088510498591No Hit
ATGGCAATTAAACACACCACTATCGACATCG6450.16042621140443822No Hit
GAGAAACCGTACGATCCGTTCGACCGCAGCC5940.14774134817711054No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5440.13530520775816185No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5300.13182308844085622No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5200.12933586035706646No Hit
TACATCCTGAAAAAAGGTGAAGAACCGTACG5060.12585374103976082No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5000.12436140418948698No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4860.12087928487218136No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4790.11913822521352853No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4780.11888950240514955No Hit
ACCGACCTGATTAGCGAAAAATACTACATCC4630.11515866027946495No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4570.1136663234291911No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG4310.10719953041133778No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4210.10471230232754804No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4200.10446357951916907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)