FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432309
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG29250.6765993768346252No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT28160.6513859299713862No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG24440.565336368199598No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22000.5088952577901454No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21320.4931657680039046No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG19790.4577744159848626No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19240.445052034540109No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17130.39624435299750876No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15670.3624722131623445No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14430.33378902590508175No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14270.3300879694847898No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14250.3296253374322533No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14230.3291627053797168No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13280.30718768288423326No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12590.2912268770717242No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12320.28498134436248146No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG12280.28405608025740847No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12030.27827317960070225No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11920.27572870331175153No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11240.2599992135255107No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11150.2579173692890964No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11100.25676078915775524No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10380.24010603526644136No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10300.23825550705629536No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG9990.2310847102419797No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9620.2225260172700545No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9550.22090680508617677No Hit
TTCGTCGGTCTCTTCCAGACGACGACCCGGAGACATACGGGCAG9280.21466127237693408No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9210.21304206019305635No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG9100.2104975839041056No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9030.20887837172022788No Hit
CATACGGTCCAGGTCTTCGGTCGCGGAGCGAGCACGGGTGCTGG8930.2065652114575454No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8910.20610257940500892No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8720.2017075749059122No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8520.19708125438054727No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8480.19615599027547423No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8260.1910670376975728No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG8160.1887538774348903No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8050.1862094011459396No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT7620.17626281201640492No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7450.17233043956984473No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7320.1693233312283575No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7070.1635404305716513No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6970.1612272703089688No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6820.1577575299149451No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6650.15382515746838488No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6580.15220594528450715No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6510.15058673310062942No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6500.15035541707436115No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6500.15035541707436115No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG6410.14827357283794693No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG6170.14272198820750898No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5970.13809566768214404No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG5660.13092487086782834No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5500.12722381444753636No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5500.12722381444753636No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5400.12491065418485389No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5300.12259749392217142No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5280.1221348618696349No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5230.12097828173829367No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5170.11959038558068419No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5090.11773985737053821No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG4990.11542669710785573No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG4910.11357616889770974No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG4850.11218827274010025No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4770.11033774452995426No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA4760.11010642850368602No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG4710.10894984837234477No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4650.1075619522147353No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG4580.10594274003085755No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4570.10571142400458931No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4500.10409221182071157No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT4450.10293563168937032No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG4410.10201036758429735No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT4380.1013164195054926No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT4350.10062247142668786No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG4350.10062247142668786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)