FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432309
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69841.6155111274574436No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49171.1373809011609752No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29710.6872399140429647No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28560.6606385710221162No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG27440.6347311760800723No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG23210.5368844969686035No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG18900.4371872896469886No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17720.4098919985473353No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16550.38282802347395034No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16510.38190275936887735No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16280.3765824907647076No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15580.3603903689259303No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15250.3527569400590781No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14700.34003455861432447No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13210.30556847070035553No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12840.29700977772843035No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12710.29400266938694314No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12510.28937634886157815No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA12510.28937634886157815No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12460.28821976873023697No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12440.2877571366777004No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA12110.2801237078108483No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11560.2674013263660946No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11290.26115579365685193No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11110.25699210518402343No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10120.23409181858346692No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9890.22877154997929722No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC9810.22692102176915122No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9510.21998154098110378No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9430.21813101277095784No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9420.21789969674468954No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9360.21651180058708006No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9250.21396732429812934No Hit
ACCCTGGAACCGGTATCCCGCATTCGTTCTC8770.20286415503725344No Hit
ATGTCTTCTCGCCGCCGTAGCTCTTCCCGTC8730.20193889093218045No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8710.201476258879644No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8410.19453677809159653No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8380.1938428300127918No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8220.1901417735924998No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8100.18736598127728085No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7990.1848215049883301No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7990.1848215049883301No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7910.18297097677818414No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7610.17603149599013668No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7600.17580017996386843No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7060.16330911454538305No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7000.16192121838777357No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6820.1577575299149451No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6710.15521305362599436No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6570.1519746292582389No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6400.14804225681167868No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6310.14596041257526446No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6270.14503514847019144No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6160.14249067218124073No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6090.140871459997363No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6050.13994619589229002No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5830.13485724331438856No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5650.1306935548415601No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5440.12583591828992688No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5410.12514197021112214No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5310.12282880994843966No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5200.12028433365948892No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5100.11797117339680645No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG4980.11519538108158747No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4830.11172564068756376No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG4820.11149432466129551No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4800.11103169260875902No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4770.11033774452995426No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4760.11010642850368602No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA4740.10964379645114952No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4660.10779326824100353No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4650.1075619522147353No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4620.10686800413593056No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4540.10501747592578457No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4500.10409221182071157No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG4480.10362957976817508No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA4420.1022416836105656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)