FastQCFastQC Report
Sun 21 May 2023
EGAF00007926515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470500
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG31860.6771519659936238No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT30760.6537725823591923No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG26560.5645058448459086No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23270.4945802337938363No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23140.491817215727949No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21350.4537725823591923No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG20360.4327311370882041No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18010.38278427205100957No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16370.3479277364505845No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15520.32986184909670563No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15370.32667375132837406No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG15340.32603613177470775No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15150.3219978746014878No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT15120.32136025504782145No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG13480.2865037194473964No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13400.28480340063761955No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13070.2777895855472901No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12970.27566418703506906No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG12530.26631243358129647No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12480.265249734325186No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12440.26439957492029753No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG12240.2601487778958555No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12130.25781083953241235No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT12060.2563230605738576No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG10860.23081827842720512No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG10550.22422954303931986No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10320.21934112646121148No Hit
TTCGTCGGTCTCTTCCAGACGACGACCCGGAGACATACGGGCAG10240.21764080765143465No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10190.21657810839532413No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10140.21551540913921358No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG10010.21275239107332622No Hit
CATACGGTCCAGGTCTTCGGTCGCGGAGCGAGCACGGGTGCTGG9640.2048884165781084No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9500.20191285866099895No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG9460.2010626992561105No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9360.1989373007438895No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9260.19681190223166845No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9130.1940488841657811No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8900.1891604675876727No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8770.18639744952178533No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8720.18533475026567484No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8180.1738575982996812No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8160.173432518597237No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7790.16556854410201913No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG7770.16514346439957492No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT7710.1638682252922423No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT7650.16259298618490967No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7350.15621679064824653No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7160.15217853347502655No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG7150.15196599362380445No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6820.14495217853347503No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6760.1436769394261424No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6760.1436769394261424No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6710.14261424017003188No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6620.14070138150903294No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6420.13645058448459085No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG6180.13134962805526038No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5970.12688629117959618No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5940.12624867162592984No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5790.1230605738575983No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5780.1228480340063762No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5670.12051009564293305No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5640.11987247608926674No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG5240.11137088204038258No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5200.11052072263549415No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT5180.11009564293304995No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5180.11009564293304995No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5180.11009564293304995No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT5140.10924548352816152No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG5130.10903294367693941No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5020.1066950053134963No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT4990.10605738575982997No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG4980.10584484590860786No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG4970.10563230605738576No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG4910.10435706695005313No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4850.1030818278427205No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4840.10286928799149841No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG4780.10159404888416577No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4770.10138150903294368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)