FastQCFastQC Report
Sun 21 May 2023
EGAF00007926516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470500
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55311.175557917109458No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG31850.6769394261424017No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31380.6669500531349628No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG30860.6558979808714134No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG26340.5598299681190223No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19810.42104144527098836No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG19700.41870350690754515No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18270.3883103081827843No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17770.37768331562167906No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17350.3687566418703507No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16930.35982996811902235No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16790.3568544102019129No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16620.35324123273113706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15610.33177470775770457No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA15000.3188097768331562No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14690.31222104144527096No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14660.3115834218916047No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14110.29989373007438896No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14110.29989373007438896No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13730.291817215727949No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13570.2884165781083953No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13230.2811902231668438No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13000.27630180658873543No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA12910.27438894792773644No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12600.26780021253985126No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12000.255047821466525No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10960.23294367693942614No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10950.23273113708820403No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10920.23209351753453772No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10830.23018065887353878No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10700.22741764080765142No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10200.21679064824654623No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10070.21402763018065887No Hit
ATGTCTTCTCGCCGCCGTAGCTCTTCCCGTC10000.21253985122210414No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9740.20701381509032943No Hit
ACCCTGGAACCGGTATCCCGCATTCGTTCTC9730.20680127523910732No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9500.20191285866099895No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9320.19808714133900104No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9280.19723698193411265No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9210.1957492029755579No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9200.1955366631243358No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9120.19383634431455896No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9100.19341126461211477No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8640.18363443145589797No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG8160.173432518597237No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8160.173432518597237No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7950.1689691817215728No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7740.1645058448459086No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7550.16046758767268862No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA7480.1589798087141339No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7460.15855472901168968No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7370.15664187035069074No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7260.15430393198724762No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7180.1526036131774708No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6920.14707757704569607No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6910.14686503719447397No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6850.14558979808714134No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC6260.13304994686503718No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6240.132624867162593No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5930.12603613177470777No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5890.12518597236981935No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5830.12391073326248672No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5780.1228480340063762No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5550.11795961742826779No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA5470.11625929861849096No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5470.11625929861849096No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5450.11583421891604676No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5330.1132837407013815No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5330.1132837407013815No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5260.11179596174282677No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA5250.11158342189160468No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG5250.11158342189160468No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5050.1073326248671626No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5030.10690754516471838No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5030.10690754516471838No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5020.1066950053134963No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4860.10329436769394261No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4850.1030818278427205No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4750.10095642933049948No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4730.10053134962805525No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4710.10010626992561106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)